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Transplantation‐Induced Functional/Morphological Changes in Rat Aorta Allografts Differ from Those in Arteries of Rat Kidney Allografts
Author(s) -
Andriambeloson Emile,
Cannet Catherine,
Pally Charles,
Klanke Bernd,
Bruns Christian,
Zerwes and HansGünter,
Bigaud Marc
Publication year - 2004
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1046/j.1600-6143.2003.00319.x
Subject(s) - medicine , aorta , kidney transplantation , transplantation , kidney , pathology , cardiology
The functional/morphological changes observed in rat aorta allografts were compared with those seen in the arteries of rat kidney allografts. Untreated allografts (F344‐to‐LEW) were collected at various times post‐transplantation (Tx). Vascular smooth muscle cell (SMC) constriction to phenylephrine (Phe) and endothelial cell (EC)‐dependent relaxation to acetylcholine (Ach) were assessed. Neointima formation in graft vessels was assessed by histology. In aorta allografts, the effects of Phe and Ach were irreversibly abolished within 3–2 weeks post‐Tx. Neointima formation was consistently detected between 4 and 8 weeks post‐Tx. In kidney allografts, sign of vasculopathy was seen in 10, 30 and 40% of resistance arteries at 8, 16 and 33 weeks post‐Tx, respectively. In the main renal artery, substantial neointima formation was not apparent before 33 weeks post‐Tx, the vasoconstrictor effect of Phe was fully maintained until then, and Ach‐induced vasorelaxation was irreversibly reduced by approximately 70% from week 2 post‐Tx onwards. These results indicate that the post‐Tx functional/morphological changes seen in aorta allografts do not reflect those seen in arteries of kidney allografts. Hence, renal arteries from rat kidney allografts can be considered as a more relevant model to study the cascade of events leading to Tx‐induced CGA in solid organ allografts.