Premium
Identification of a coffee berry borer‐associated yeast: does it break down caffeine?
Author(s) -
Vega Fernando E.,
Blackburn Michael B.,
Kurtzman Cletus P.,
Dowd Patrick F.
Publication year - 2003
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1046/j.1570-7458.2003.00034.x
Subject(s) - biology , berry , caffeine , yeast , botany , pest analysis , horticulture , food science , genetics , endocrinology
Two yeasts isolated from laboratory reared adult coffee berry borers [ Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae)] and from insects collected in the field in Colombia were identified as Pichia burtonii Boidin and Candida fermentati (Saito) Bai, based on sequencing of the nuclear large subunit 26S rDNA variable D1/D2 domain. Liquid culture experiments using P. burtonii in media containing different caffeine levels indicated that caffeine levels in a range found within coffee seeds can retard yeast growth. HPLC analysis shows that P. burtonii does not break down caffeine.