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Transgenic rice plants expressing a trypsin inhibitor are resistant against rice stem borers, Chilo suppressalis
Author(s) -
Mochizuki Atsushi,
Nishizawa Yaeko,
Onodera Haruko,
Tabei Yutaka,
Toki Seiichi,
Habu Yoshiki,
Ugaki Masashi,
Ohashi Yuko
Publication year - 1999
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1046/j.1570-7458.1999.00576.x
Subject(s) - chilo suppressalis , biology , pyralidae , genetically modified rice , genetically modified crops , lepidoptera genitalia , oryza sativa , pest analysis , cnaphalocrocis medinalis , transgene , botany , trypsin inhibitor , trypsin , gene , biochemistry , enzyme
A synthetic gene, mwti1b , coding for a winged bean trypsin inhibitor WTI‐1B, has been introduced and expressed in rice plants, Oryza sativa . Protein extracts from transgenic rice plants expressing the trypsin inhibitor inhibited the gut proteases of larvae of the serious insect pest, the rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) in vitro . The growth of larvae reared on transgenic rice plants expressing WTI‐1B at more than 1 ng/10 μg total protein was significantly retarded compared to that on non‐transgenic control plants.