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Allozyme variation among Brazilian and U.S. populations of Rhyzopertha dominica resistant to insecticides
Author(s) -
Guedes Raul Narciso C.,
Kambhampati Srinivas,
Dover Barry A.
Publication year - 1997
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1046/j.1570-7458.1997.00197.x
Subject(s) - biology , bostrichidae , pest analysis , insecticide resistance , genetic variation , genetic variability , pesticide resistance , resistance (ecology) , population genetics , ecology , toxicology , population , pesticide , botany , genotype , demography , genetics , sociology , gene
The lesser grain borer ( Rhyzopertha dominica (F.)) is an important pest of stored grain in many parts of the world (Paleartic, Ethiopian, Oriental, Australian, Neotropical, and Neartic regions) with the ability to fly long distances. These insects have been shown to be resistant to organophosphorus insecticides in several studies. Polyacrylamide gel electrophoresis was used to assess the genetic variability within and among eight Brazilian and seven United States populations of R. dominica and to determine how insecticide resistance may be spreading within both countries. Significant variation in allele frequency among populations was observed at all six polymorphic enzyme loci that were examined. The Brazilian and U.S. populations were genetically differentiated from one another; populations within the U.S. and those within Brazil were also differentiated from one another. The mean genetic similarity among the seven U.S. populations collected in a small region in northeast Kansas was smaller than that among eight Brazilian populations collected in a relatively large geographical area. These results are consistent with the resistance ratios to chlorpyriphos‐methyl in R. dominica populations from Brazil and the U.S. and the information available concerning patterns of flight activity in this insect.