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Specific detection of human coagulation factor IX in cynomolgus macaques
Author(s) -
Mimuro J.,
Mizukami H.,
Ono F.,
Madoiwa S.,
Terao K.,
Yoshioka A.,
Ozawa K.,
Sakata Y.
Publication year - 2004
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1046/j.1538-7933.2003.00572.x
Subject(s) - monoclonal antibody , macaque , antibody , epitope , immunoassay , human plasma , microbiology and biotechnology , rhesus macaque , coagulation , biology , virology , chemistry , immunology , medicine , chromatography , paleontology
Summary. After screening for species‐specific antihuman factor (F)IX monoclonal antibodies, we found that antibody 3A6 did not bind to cynomolgus FIX. The 3A6 epitope was found to include Ala262 of human FIX. The 3A6 antibody was used as a catching antibody in an enzyme immunoassay (EIA) for specific detection of human FIX in cynomolgus macaque plasma. No significant increase of substrate hydrolysis was observed when EIA buffer containing cynomolgus macaque plasma was subjected to the 3A6‐based EIA. Addition of up to 30% cynomolgus macaque plasma or canine plasma to the assay did not alter detection of human FIX. Three cynomolgus macaques were injected with human FIX (10 U kg −1 ; i.v.) and the circulating human FIX was quantified in the macaque plasma. The FIX level in the circulation increased to 470 ± 37.6 ng mL −1 at 1 h after the injection and gradually decreased to 1.79 ± 1.1 ng mL −1 by day 5, which is approximately 0.06% of the normal human plasma FIX concentration. These data suggest that the cynomolgus macaque can be used as a primate model for studying hemophilia B gene therapy by transduction of macaque organs with vectors to express human FIX in vivo and detection of human FIX using the 3A6 monoclonal antibody.