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FGF‐2 but not FGF‐1 binds fibrin and supports prolonged endothelial cell growth
Author(s) -
Sahni A.,
Altland O. D.,
Francis C. W.
Publication year - 2003
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1046/j.1538-7836.2003.00250.x
Subject(s) - fibrin , fibroblast growth factor , cell growth , endothelial stem cell , chemistry , growth factor , microbiology and biotechnology , cell , fibrinogen , biology , immunology , biochemistry , in vitro , receptor
Summary. Endothelial cell viability and growth are dependent on both polypeptide growth factors, and integrin‐mediated matrix interactions. We have now examined the ability of fibrin‐binding and non‐binding growth factors to support long‐term endothelial cell growth in the presence or absence of the soluble form. Endothelial cells were cultured on a fibrin surface, with or without FGF‐1 or FGF‐2, and proliferation was determined by 3 H‐thymidine incorporation. Cells cultured on fibrin with no growth factor showed minimal proliferation up to 96 h. In contrast, when FGF‐2 was incorporated into fibrin, proliferation was increased 6.5 ± 0.6‐fold, equal to growth on a fibrin surface with FGF‐2 continually present in the medium. Thymidine incorporation was similar when cells were cultured on a fibrin surface that had been incubated with FGF‐2 and then the growth factor removed (8.6 ± 0.5‐fold). In contrast to results with FGF‐2, a surface of fibrin exposed to FGF‐1 supported minimal growth, whereas growth was comparable to either FGF‐1 or FGF‐2 present in the medium. Comparable results were observed when proliferation was quantitated by cell counting at times up to 48 h. Binding studies demonstrated no high‐affinity interaction of FGF‐1 with fibrinogen or fibrin. We conclude that FGF‐2 bound to fibrin supports prolonged endothelial cell growth as well as soluble FGF‐2, whereas FGF‐1 does not bind to fibrin and can support endothelial cell growth only if continually present in soluble form. Fibrin may serve as a matrix reservoir for FGF‐2 to support cell growth at sites of injury or thrombosis.