Premium
CXC‐chemokines in coronary artery disease: possible pathogenic role of interactions between oxidized low‐density lipoprotein, platelets and peripheral blood mononuclear cells
Author(s) -
Holm T.,
Damås J. K.,
Holven K.,
Nordøy I.,
Brosstad F. R.,
Ueland T.,
Währe T.,
Kjekshus J.,
Frøland S. S.,
Eiken H. G.,
Solum N. O.,
Gullestad L.,
Nenseter M.,
Aukrust P.
Publication year - 2003
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1046/j.1538-7836.2003.00065.x
Subject(s) - peripheral blood mononuclear cell , platelet , peripheral , chemokine , medicine , coronary artery disease , lipoprotein , peripheral blood , immunology , chemistry , cardiology , inflammation , cholesterol , biochemistry , in vitro
Summary. CXC‐chemokines may be involved in atherogenesis. Herein we examined the possible role of CXC‐chemokines in the inflammatory interactions between oxidized (ox‐) low‐density lipoprotein (LDL), platelets and peripheral blood mononuclear cells (PBMC) in 15 patients with coronary artery disease (CAD) without ‘traditional’ risk factors and 15 carefully matched controls. Our main findings were: (a) ox‐LDL stimulated the release of the CXC‐chemokines interleukin (IL)‐8, ENA‐78 and GRO‐α from PBMC, particularly in CAD. (b) In platelets, ox‐LDL induced release of ENA‐78 and, when combined with SFLLRN, also of GRO‐α, with significantly higher response in CAD. (c) Platelet‐rich plasma, especially when costimulated with ox‐LDL, enhanced the release of IL‐8 from PBMC, particularly in CAD patients. (d) Freshly isolated PBMC showed markedly increased IL‐8 mRNA expression in CAD patients. Our findings suggest enhanced inflammatory interactions between ox‐LDL, platelets and PBMC in CAD patients involving CXC‐chemokine related mechanisms, possible contributing to atherogenesis in these and other CAD patients.