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An influence of ABO blood group on the rate of proteolysis of von Willebrand factor by ADAMTS13
Author(s) -
Bowen D. J.
Publication year - 2003
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1046/j.1538-7836.2003.00007.x
Subject(s) - proteolysis , von willebrand factor , adamts13 , chemistry , medicine , endocrinology , ristocetin , fibrinogen , platelet , microbiology and biotechnology , biochemistry , enzyme , biology
Summary. The susceptibility of von Willebrand factor (VWF) of blood group O, A, B and AB to proteolysis by the ADAMTS13 metalloprotease was investigated. Multimeric analysis indicated that the rate of VWF proteolysis differed between blood groups and was greater for group O VWF than for non‐O VWF in the rank order O ≥ B > A ≥ AB. Measurement of the collagen binding activity of VWF of each blood group following proteolysis for a fixed time interval corroborated the results obtained on multimer analysis: the loss of collagen binding activity was greater for VWF of group O compared with non‐O VWF, in the rank order O ≥ B > A ≥ AB. Ristocetin was found to increase the rate of VWF proteolysis approximately two‐fold; the differential between blood groups was retained in the presence of ristocetin.