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DAK, a new low‐incidence antigen in the Rh blood group system
Author(s) -
Reid Marion E.,
Storry Jill R.,
Sausais Laima,
Tossas Edith,
Rios Maria,
HueRoye Kim,
Gloster Elizabeth S.,
Miller Scott T.,
Wolf Carl,
LomasFrancis Christine
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00517.x
Subject(s) - antigen , antibody , rh blood group system , hemagglutination , microbiology and biotechnology , phenotype , hemagglutination assay , incidence (geometry) , immunology , virology , red blood cell , biology , isoantibodies , chemistry , genetics , gene , physics , titer , optics
BACKGROUND: Some low‐incidence antigens in the Rh blood group system (e.g., VS, Rh32, FPTT) are expressed by more than one Rh complex. We describe a new low‐incidence antigen that is present on RBCs with the partial D phenotypes, D IIIa or DOL, on N RBCs and on one example of STEM+ S RBCs. STUDY DESIGN AND METHODS: Standard hemagglutination testing was performed with two sera that agglutinated D IIIa RBCs on our in‐house antibody identification panel. DNA‐based assays were performed on selected samples. RESULTS: RBCs with the D IIIa (n = 31), DOL (n = 5), or N (n = 10) phenotype were agglutinated by both sera, as were RBCs from one STEM+ S person. Reactivity with RBCs of either D IIIa or DOL phenotypes was stronger than with N RBCs and could not be separated by adsorption and elution. CONCLUSION: An antibody, anti‐DAK, which recognizes a novel low‐incidence antigen that is more strongly expressed on D IIIa and DOL RBCs than on N RBCs is described. The antibody agglutinated RBCs from 4 percent of D+ African American blood donors in New York. The antigen, DAK, has been assigned the ISBT number RH54 (004.054).