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Hematologic recovery after autologous PBPC transplantation: importance of the number of postthaw CD34+ cells
Author(s) -
Feugier Pierre,
Bensoussan Daniele,
Girard Florence,
Alla Francois,
Schuhmacher Anne,
LatgerCannard Veronique,
Hulin Cyrille,
Witz Francis,
Witz Brigitte,
Carret Anne Sophie,
Bordigoni Pierre,
Lederlin Pierre,
Janot Christian
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00446.x
Subject(s) - biology , transplantation , cd34 , andrology , cryopreservation , stem cell , medicine , embryo , genetics
BACKGROUND: The implementation of a quality‐assurance program is a major requirement to ensure quality and safety of the final PBPC components intended for clinical use. It is not clear whether the quantification of CFU‐GM and CD34+ cells should be done on fresh components and after cryopreservation, which better represents the actual composition of the graft. STUDY DESIGN AND METHODS: Correlation between prefreeze and postthaw MNCs, CD34+ cells, and CFU‐GM collected from 126 patients undergoing BMT (n=43) or PBPC (n =83) transplantation were evaluated. The statistical incidence of prefreeze and postthaw parameters as well as patient characteristics and conditioning regimens on hematologic recovery were analyzed. RESULTS: By multivariate analysis, prefreeze and postthaw CD34+ cells were the only two variables significantly and independently correlated to hematologic recovery. Low prefreeze and postthaw CD34+ cell numbers associated to a low CD34+ yield characterize PBPC grafts from patients who have the slowest hematologic recovery. The postthaw PBPC CD34+ cell number can be estimated before conditioning regimen by thawing a small aliquot of the graft. CONCLUSION: In association to prefreeze CD34+ cell number and to CD34+ yield, postthaw CD34+ cell number may be useful in monitoring cell loss during processing and identifying patients at risk of slow PBPC engraftment.