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Changes in functional activities of plasma fibrinogen after treatment with methylene blue and red light
Author(s) -
Suontaka AnnaMaija,
Blombäck Margareta,
Chapman John
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00377.x
Subject(s) - fibrin , fibrinogen , fibrinolysis , chemistry , methylene blue , clotting time , coagulation , chromatography , biophysics , biochemistry , medicine , immunology , biology , photocatalysis , catalysis
BACKGROUND : Methylene blue (MB) plus light treatment used for virus inactivation of human plasma units may lead to changes in the functional activities of fibrinogen. STUDY DESIGN AND METHODS: Single‐donor units of fresh plasma were treated with 1.0 μMMB and a red light dose of 48 J per cm2 . The effects of MB plus red light treatment on fibrinogen clottability, fibrin polymerization and gelation, clot stabilization, and fibrinolysis were studied. RESULTS: The concentration of clottable fibrinogen was unchanged during MB plus red light treatment, but a light‐dose‐dependent decrease of the concentration of functional fibrinogen was found. The initial release rate of fibrinopeptide A was slightly increased after MB plus red light treatment. Turbidity measurements of fibrin gel showed prolonged clotting time, lower fibrin fiber mass‐to‐length ratio, and slightly smaller fiber diameter. At a given clotting time, a gel with lower fibrin fiber mass‐to‐length ratio was produced. Clot stability and fibrinolysis remained normal.l ‐Histidine added to plasma before MB plus red light treatment normalized the thrombin‐induced coagulation time in a dose‐dependent way. CONCLUSION : MB plus red light treatment affected the polymerization and gelation phase of fibrin. A tighter fibrin gel structure was formed. No effect on stabilization of fibrin clot or fibrinolysis was found.