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Analysis of PBPC cell yields during large‐volume leukapheresis of subjects with a poor mobilization response to filgrastim
Author(s) -
Moncada Victoria,
Bolan Charles,
Yau Yu Ying,
Leitman Susan F.
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00361.x
Subject(s) - leukapheresis , cd34 , medicine , filgrastim , mobilization , progenitor cell , andrology , granulocyte colony stimulating factor , chemotherapy , biology , stem cell , history , genetics , archaeology
BACKGROUND : The circulating CD34 count is a reliable predictor of peripheral blood progenitor cell (PBPC) yields in subjects with a vigorous mobilization response to G‐CSF, however, the value of this parameter in poor mobilizers is uncharacterized. STUDY DESIGN AND METHODS : Consecutive PBPC procedures (n = 81) with preapheresis CD34 counts less than 20 per μL (poor mobilizers) were compared with an equal number of good mobilizers (preapheresis CD34 counts ≥20/μL). G‐CSF was administered at standard doses (10 μg/kg/day). RESULTS : CD34 yields correlated strongly with preapheresis CD34 counts in both good and poor mobilizers and were higher in allogeneic than autologous donors. For a standard 75‐kg recipient, a CD34 cell dose of greater than 2 × 10 6 per kg was never achieved in less than two 15‐L procedures if the preapheresis CD34 count was less than 8 per μL. Preapheresis WBC and MNC counts were lower in poor than in good mobilizers (28.4 vs. 43.0 and 3.25 vs. 5.01 × 10 3 /μL, respectively, p < 0.0001). Total WBC counts correlated more strongly with preapheresis CD34 counts, total CD34 yields, and CD34 yields per L processed in good mobilizers (R = 0.50, R = 0.44, and R = 0.42, respectively) than in poor mobilizers (R = 0.22, R = 0.02, and R = 0.01, respectively), whereas total MNC counts correlated more strongly with these parameters in poor (R = 0.38, R = 0.23, and R = 0.27, respectively) than in good mobilizers (R = 0.04, R = 0.13, and R = 0.16, respectively). CONCLUSION : CD34 cell yields correlate strongly with preapheresis CD34 counts. Based on this analysis, a CD34 count greater than or equal to 8 per μL is the threshold for performing PBPC collections in our institution.