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Seven‐day storage of apheresis platelets: report of an in vitro study
Author(s) -
Dumont Larry J.,
VandenBroeke Tania
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00286.x
Subject(s) - platelet , apheresis , chemistry , plateletpheresis , zoology , medicine , andrology , biology
BACKGROUND : The objective of this study was to determine the allowable platelet content limits for apheresis platelets stored for 7 days in a platelet storage bag (COBE ELP, Gambro BCT). METHODS : Apheresis platelets under controlled concentration and volume per bag were stored in plasma up to 8 days at 22°C with horizontal agitation. Routinely evaluated in vitro platelet parameters were followed. Oxygen consumption was directly measured with a Clark‐type electrode. All components were cultured in aerobic medium on Day 7. RESULTS: Twenty‐four components were evaluated in storage configurations (median [range], 340 [110‐402] mL, 1.32 [0.99‐2.45]× 106platelets/μL, and 4.8 [1.4‐5.9]× 1011platelets/bag). No bacterial contamination was detected. One component had a pH value at 22°C of below 6.0 before Day 5 with attendant loss of all other in vitro function measures. The pH value at 22°C was maintained above 6.2 for the remaining 23 components. A pH value of greater than 7.4 was observed at some point in storage for 13 of 23 units, although platelet function or activation was not adversely affected. Aerobic metabolic function was maintained over 7 days with O2consumption of 321 μmol per hour per 1012 platelets on Day 7. CONCLUSION: Although a continuing decline of platelet in vitro characteristics can be observed for storage beyond 5 days, apheresis platelets in plasma stored 100 to 400 mL per bag, 1.0 × 106to 2.5 × 106platelets per μL, and a maximum of 5.1 × 1011 platelets per bag maintained in vitro platelet characteristics over 7 days of storage.