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Long‐term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation: a comparison between controlled‐rate freezing and uncontrolled‐rate freezing at 80°C
Author(s) -
Montanari Mauro,
Capelli Debora,
Poloni Antonella,
Massidda Danilo,
Brunori Marino,
Spitaleri Luca,
Offidani Massimo,
Lucesole Moira,
Masia Maria C.,
Balducci Florinda,
Refe Cristina,
Piani Mario,
Leoni Pietro,
Olivieri Attilio
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00271.x
Subject(s) - cryopreservation , cryoprotectant , medicine , transplantation , progenitor cell , peripheral blood , platelet , andrology , stem cell , surgery , biology , embryo , genetics , microbiology and biotechnology
BACKGROUND : The most widely used system for peripheral blood progenitor cell (PBPC) cryopreservation is controlled‐rate freezing (CRF). Uncontrolled‐rate freezing (URF) at –80°C has also been used, but its clinical impact has not been studied sufficiently yet. STUDY DESIGN AND METHODS : Two groups of patients were compared: Group A consisted of 69 patients autotransplanted with PBPCs cryopreserved with CRF; Group B consisted of 192 patients autotransplanted with PBPCs cryopreserved with URF at –80°C. The same cryoprotectant solution and storage system were used. RESULTS: A significant delay of hematologic reconstitution (HR) in the URF group was observed for neutrophils greater than 0.5 × 109per L and for platelets greater than 20 × 109per L and greater than 50 × 109 per L; we did not observe any differences in the clinical course. The long‐term HR was comparable in the two groups, all patients showed stable engraftment, and no late graft failures were observed. CONCLUSION : Our study confirms that URF is safe and allows sustained long‐term engraftment without increasing the risks of transplantation, even though the early engraftment after URF is slower.