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Screening for alloantibodies in the serum of patients receiving platelet transfusions: a comparison of the ELISA, lymphocytotoxicity, and the indirect immunofluorescence method
Author(s) -
Levin MarkDavid,
De Veld Jos C.,
Van der Holt Bronno,
Van't Veer Mars B.
Publication year - 2003
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2003.00254.x
Subject(s) - medicine , platelet , immunofluorescence , screening test , immunology , gastroenterology , platelet transfusion , antibody , pediatrics
BACKGROUND : For screening of alloimmunization in patients repeatedly receiving platelet transfusions, different tests are used, none of which is the standard. Here we describe a comparison of four tests most commonly used for detection of alloimmunization in a group of nonselected patients receiving platelet transfusions. STUDY DESIGN AND METHODS : In 99 patients with hematologic malignancies who received platelet transfusions, 192 random serum samples were tested in the ELISA, the lymphocytotoxic test (LCT), the lymphocyte immunofluorescence test (LIFT), and the platelet immunofluorescence test (PIFT). Results of all tests were compared. RESULTS : The results of all tests were significantly correlated with each other (p < 0.005). ELISA and LIFT were more often positive than LCT and PIFT. ELISA and LIFT showed the best correlation (chi‐square test = 63.7, p < 0.001). CONCLUSION : ELISA, the least time‐consuming test, detects alloimmunization as often as LIFT and more often than LCT and PIFT.