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Combined isolation of CD34+ progenitor cells and reduction of B cells from peripheral blood by use of immunomagnetic methods
Author(s) -
MartínHenao G.A.,
Picón M.,
Rueda F.,
Amill B.,
Querol S.,
GonzalezBarca E.,
Ferrá C.,
Grañena A.,
García J.
Publication year - 2002
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2002.00146.x
Subject(s) - immunomagnetic separation , cd19 , cd34 , progenitor cell , chronic lymphocytic leukemia , follicular lymphoma , haematopoiesis , b cell , microbiology and biotechnology , immunology , medicine , lymphoma , antibody , stem cell , biology , leukemia , genetics
BACKGROUND : Malignant cells may contribute to relapse after autologous hematopoietic cell transplantation The effectiveness of a double immunomagnetic purging strategy combining CD34‐positive with B‐negative cell selection to purge peripheral blood progenitor cells (PBPCs) from patients with chronic lymphoproliferative disorders has been analyzed. STUDY DESIGN AND METHODS : Twenty‐two CD34+ cell selections from patients with follicular lymphoma (n = 14), chronic lymphocytic leukemia (n = 6), mantle cell lymphoma (n = 1), and splenic marginal zone lymphoma (n = 1) were performed by use of a magnetic cell selector followed by a negative cell selection step with anti‐CD19 monoclonal antibody bound to immunomagnetic beads. RESULTS : The PBPC components contained median CD34+ cells of 1.24 percent (range, 0.38‐3.92%) and CD19+ cells of 1.83 percent (range, 0.06‐69.7%). After positive selection (n = 22), 49 percent (range, 16‐72%) of CD34+ cells were recovered with a purity of 93 percent (range, 24‐99%). The double‐positive and ‐negative selections (n = 20) yielded 57.5 percent of CD34+ cells (range, 33.4‐79.4%) with a purity of 95 percent (range, 63‐99%). Logarithms of B‐cell reduction in the CD34+‐cell‐enriched B‐cell‐depleted component had a median value of 3.63 (range, 2.74‐4.84 log) and CD19+ and CD5+ cells for chronic lymphocytic leukemia patients with more than 4.56 log (>3.6‐5.6 log). Of 13 PBPC components that had a tumor‐specific clonal signal, 10 became PCR negative after the double‐selection procedure. CONCLUSION : Combined positive and negative magnetic cell selection achieves a high grade of tumor cell reduction with up to 77 percent of the grafts being negative for tumor‐specific clonal signal by PCR analysis. This technique preserves an adequate recovery of progenitor cells able to engraft.