Premium
Evaluation of a new generation of culture bottle using an automated bacterial culture system for detecting nine common contaminating organisms found in platelet components
Author(s) -
Brecher M.E.,
Heath D.G.,
Hay S.N.,
Rothenberg S.J.,
Stutzman L.C.
Publication year - 2002
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2002.00122.x
Subject(s) - microbiology and biotechnology , staphylococcus epidermidis , enterobacter cloacae , anaerobic exercise , propionibacterium acnes , klebsiella oxytoca , aerobic bacteria , serratia marcescens , staphylococcus aureus , biology , staphylococcus , bacillus cereus , bacteria , escherichia coli , enterobacteriaceae , physiology , biochemistry , genetics , gene
BACKGROUND : An automated bacterial culture system (BacT/ALERT 3D, bioMérieux) has been previously validated with a variety of bacteria in platelets. The recovery of bacteria in platelets using a new generation of culture bottles that do not require venting and that use a liquid emulsion sensor was studied. STUDY DESIGN AND METHODS:Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, Streptococcus viridans,andPropionibacterium acnes isolates were inoculated into Day 2 platelets to concentrations of 10 and 100 CFU per mL. Samples were then studied with current and new aerobic, anaerobic, and pediatric bottles. RESULTS: All organisms, exceptP. acnes,were detected in a mean time of 9.2 to 20.4 (10 CFU/mL) or 8.7 to 18.6 (100 CFU/mL) hours.P. acnes was detected in a mean time of 69.2 (10 CFU/mL) or 66.0 (100 CFU/mL) hours. The 10‐fold increase in inoculum was associated with a mean 9.2 percent difference in detection time. The aerobic, anaerobic, and pediatric bottles had a mean difference in detection time (hours) between the current and new bottles of 0.10 (p = 0.61), 0.4 (p = 0.38), and 1.0 (p < 0.001), respectively. CONCLUSION : No difference in detection time between the current and new aerobic and anaerobic bottles was demonstrated. The new pediatric bottles had a small but significant delay in detection.