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Volume of RBCs, 24‐ and 48‐hour posttransfusion survivals, and the lifespan of 51 Cr and biotin‐X‐N‐hydroxysuccinimide (NHS)‐labeled autologous baboon RBCs: effect of the anticoagulant and blood pH on 51 Cr and biotin‐X‐NHS elution in vivo
Author(s) -
Valeri C. Robert,
Pivacek Linda E.,
Cassidy George P.,
Ragno Gina
Publication year - 2002
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2002.00071.x
Subject(s) - in vivo , biotin , anticoagulant , baboon , chemistry , blood volume , red blood cell , whole blood , heparin , chromatography , medicine , biochemistry , biology , microbiology and biotechnology
BACKGROUND: The RBC injury that occurs during collection of the first few milliliters of blood into the pH 5.0 ACD (NIH, Formula A) is referred to as the lesion of collection. The RBC injury was evaluated by labeling the ACD RBCs with 51 Cr and measuring the 24‐hour posttransfusion survival. The effect of the acidification of ACD blood on the in vivo elution of 51 Cr from the RBC has not been reported. STUDY DESIGN AND METHODS: Baboon blood was collected in heparin and in ACD and CP2D at different ratios of blood to anticoagulant. ACD blood with a pH of 5.7 to 6.9 was labeled with 51 Cr. Heparinized blood with a pH of 7.4 was labeled with biotin‐X‐N‐hydroxysuccinimide (NHS). ACD blood with a pH of 5.9 was labeled with both 51 Cr and biotin‐X‐NHS. The RBC volumes, 24‐ and 48‐hour posttransfusion survivals, and lifespans (T50) were measured. RESULTS: The RBC volume of ACD blood with a pH ranging from 5.7 to 6.9 was not affected by 51 Cr labeling. 51 Cr‐labeled ACD blood with a pH of 6.9 had an RBC volume that was significantly greater than that seen in heparinized blood with a pH of 7.4 labeled with biotin‐X‐NHS. In vivo elution of 51 Cr from the RBCs prepared from the ACD blood with a pH of 5.7 to 6.9 and in vivo elution of biotin‐X‐NHS from RBCs prepared from ACD blood with a pH of 5.9 were associated with reductions in 24‐ and 48‐hour posttransfusion survivals and T50. CONCLUSIONS: The anticoagulant and the pH of the medium in which the RBCs were labeled with 51 Cr or biotin‐X‐NHS affected in vivo elution of the label from the RBCs and may reduce posttransfusion survival values.