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Storage of platelets in additive solutions: effects of magnesium and/or potassium
Author(s) -
De WildtEggen Janny,
Schrijver Johanna G.,
Bins Maarten,
Gulliksson Hans
Publication year - 2002
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2002.00012.x
Subject(s) - chemistry , magnesium , potassium , platelet , sodium , mole , nuclear chemistry , biochemistry , medicine , organic chemistry
BACKGROUND: Previous studies indicate that platelet concentrates (PCs) in a platelet additive solution (PAS) containing citrate, acetate, and sodium chloride (PAS‐2) show a significantly higher increase of CD62+ platelets than PCs in other brands of PAS containing Mg 2+ and K + . To investigate whether this difference can be explained by the presence of Mg 2+ and/or K + in the storage medium, we performed paired studies comparing storage of PCs in PAS‐2 to PAS‐2 with either Mg 2+ or K + or both in combination. STUDY DESIGN AND METHODS: PCs from pooled buffy coats were prepared in either PAS‐2 or PAS‐2 with Mg 2+ or K + or both in combination (PAS‐2 modified). Different volumes of MgCl 2 solution (1 mol/L) and/or KCl solution (1 mol/L) were added to PAS‐2 to obtain various concentrations. After preparation and during storage (at Days 3 and 7), pH, pCO 2 , pO 2 , HCO 3 − , and CD62 (%) were measured. RESULTS: During 7 days of storage, pH was very stable (6.9‐7.2) in all PCs. At Day 7, platelet CD62 expression was 49 percent (PAS‐2), 41 percent (PAS‐2 with 1.5 mmol/L Mg 2+ ), and 38 percent (PAS‐2 with 4.5 mmol/L Mg 2+ ). With added K + , at Day 7, expression of CD62 was 55 percent (PAS‐2), 39 percent (PAS‐2 with 4.5 mmol/L K + ), and 35 percent (PAS‐2 with 9.0 mmol/L K + ). In PAS‐2 modified (PAS‐2 with 1.5 mmol/L Mg 2+ and 4.5 mmol/L K + ) and CPD plasma, the corresponding CD62 values were 23 and 35 percent, respectively. CONCLUSION: The combination of Mg 2+ and K + gave significantly (p < 0.05) lower platelet CD62 expression in the storage medium than in PAS‐2. The effects of these differences on platelet metabolism and in vivo properties remain to be investigated.