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RBC storage for 11 weeks
Author(s) -
Hess John R.,
Rugg Neeta,
Gormas Jenny K.,
Knapp Amy D.,
Hill Heather R.,
Oliver Cynthia K.,
Lippert Lloyd E.,
Silberstein Edward B.,
Greenwalt Tibor J.
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41121586.x
Subject(s) - hemolysis , mannitol , microvesicle , in vivo , medicine , chemistry , andrology , biology , immunology , biochemistry , microbiology and biotechnology , microvesicles , microrna , gene
BACKGROUND: Increasing the length of RBC storage can increase both RBC availability and quality. This work addresses 11‐week RBC storage in experimental ASs (EASs). STUDY DESIGN AND METHODS: Three studies were performed. In the first, 24‐hour in vivo recovery of 51 Crlabeled autologous RBCs was measured in nine volunteers after storage of their RBCs for 11 weeks in EAS 67. In the second study, 4 units of blood were divided and stored in aliquots with an EAS containing 0, 15, 30, or 45 mmol per L of mannitol; then hemolysis, RBC morphology, and microvesicle protein were measured. In the third study, 6 full units were stored for 12 weeks in the EAS containing 30 mmol per L of mannitol, with weekly sampling for morphologic and biochemical measures of RBC quality. RESULTS: RBCs stored for 11 weeks in EAS‐67 had a mean 24‐hour in vivo recovery of 79 ± 5 percent, but the hemolysis was 1.35 ± 0.68 percent. Increasing mannitol content of the EAS reduced hemolysis but increased microvesiculation. EAS‐76, with 30 mmol per L of mannitol allowed 11‐week storage with 0.48 ± 0.10 percent hemolysis at 11 weeks and 0.62 ± 0.14 percent hemolysis at 12 weeks. CONCLUSION: It is possible to store RBCs for 11 weeks in EAS with greater than 75 percent recovery and less than 1 percent hemolysis.