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Duck hepatitis B photoinactivation bydimethylmethylene blue in RBC suspensions
Author(s) -
Wagner Stephen J.,
Skripchenko Andrey,
Pugh John C.,
Suchmann Donna B.,
Ijaz M. Khalid
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41091154.x
Subject(s) - chemistry , duck hepatitis b virus , biology , virus , hepatitis b virus , hepadnaviridae , virology
BACKGROUND: Dimethylmethylene blue (DMMB) has been used to photoinactivate a number of model viruses, including VSV, in RBC suspensions under conditions that preserve in vitro RBC properties during storage. 23 The relative sensitivity of duck HBV (DHBV) and VSV to photoinactivation by DMMB was investigated by performing an indirect immunofluorescence assay (IFA) using primary duck hepatocyte (PDH) cultures or a standard plaque assay for the respective viruses. STUDY DESIGN AND METHODS: DMMB was added to 45‐percent Hct, WBC‐reduced, oxygenated AS‐3 RBCs at 10‐, 1‐, and 0.1‐μ M concentrations. Samples (1‐mm thick) were illuminated with 5.4‐mW per cm 2 of red light for 2 or 9 seconds. Unilluminated samples without DMMB or with 10 μ M DMMB served as control. RESULTS: DHBV and VSV were rapidly photoinactivated by DMMB in a concentration and light‐dose‐dependent fashion. Neither virus was substantially inactivated by incubation with DMMB in the dark. For a given light exposure, DHBV required a concentration of DMMB one‐one hundredth that of VSV to achieve approximately the same level of inactivation. CONCLUSION: DHBV appears to be considerably more sensitive than VSV to DMMB photoinactivation. Photoinactivation in 45‐percent Hct RBCs can be achieved in seconds by using micromolar quantities of dye.