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DNA analysis for the Dombrock polymorphism
Author(s) -
Rios Maria,
HueRoye Kim,
Lee Agnes H.,
Chiofolo Joseph T.,
Miller Jeffrey L.,
Reid Marion E.
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41091143.x
Subject(s) - typing , biology , genetics , asparagine , genotype , allele , restriction fragment length polymorphism , microbiology and biotechnology , polymorphism (computer science) , primer (cosmetics) , gene , amino acid , chemistry , organic chemistry
BACKGROUND: RBC typing for Do a and Do b is notoriously difficult, and inaccurate typing can predispose patients to hemolytic transfusion reactions. The DO1/DO2 polymorphism is associated with three nucleotide changes: 378 C>T, 624 T>C and 793 A>G. While the 378 C>T‐ and 624 T>C‐containing codons are silent mutations, the 793 A>G polymorphism in codon 265 encodes asparagine for Do a and aspartic acid for Do b . STUDY DESIGN AND METHODS: Described here are two PCR‐RFLP assays, one using the Mnl I site associated with 624C ( DO2 ) and the other altering two nucleotides within the sense primer, which allows recognition of 793G by the Eam 1105 I. RESULTS: The assays have been performed on over 100 samples for which the RBC typing of one or both antigens was known. Eight samples had been historically mistyped by hemagglutination. CONCLUSION: This RFLP assay provides a practical method for typing donor blood for Dombrock alleles.