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Immune reconstitution after autologous selectedperipheral blood progenitor cell transplantation:comparison of two CD34+ cell‐selection systems
Author(s) -
Laurenti Luca,
Sorà Federica,
Piccirillo Nicola,
Chiusolo Patrizia,
Cicconi Silvia,
Rutella Sergio,
Serafini Riccardo,
Garzia Maria Grazia,
Leone Giuseppe,
Sica Simona
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41060783.x
Subject(s) - cd34 , cd19 , andrology , cd3 , progenitor cell , transplantation , immunology , medicine , lymphocyte , stem cell , immune system , biology , cd8 , microbiology and biotechnology
BACKGROUND: Selection of CD34+ PBPCs has been applied as a method of reducing graft contamination from neoplastic cells. This procedure seems to delay lymphocyte recovery, while myeloid engraftment is no different from that with unselected PBPC transplants. STUDY DESIGN AND METHODS: Lymphocyte recovery was studied in two groups of patients who underwent autologous CD34+ PBPC transplant with two different technologies (Ceprate SC, Cellpro [n = 17]; CliniMACS, Miltenyi Biotech [n = 13]). The median number of CD34+ cells transfused was 3.88 × 10 6 per kg and 3.32 × 10 6 per kg, respectively. Residual CD3 cells × 10 6 per kg were 4.97 and 0.58, respectively (p = 0.041). Residual CD19 cells × 10 6 per kg were 1.33 and 0.73, respectively (NS). RESULTS: No differences were found between the two groups in total lymphocyte recovery to >0.5 × 10 9 per L, which achieved a stable count by Day 30. During the study period, the CD4+ cell count remained below 0.2 × 10 9 per L, and the B‐cell subset showed a trend toward normalization. CD3/HLA‐DR+ and CD16/56 increased markedly in both groups by Day 30. An increase in CMV (13%) and adenovirus (17.4%) infection was found in both groups. CONCLUSION: Both CD34+ cell selection technologies used here determined an excellent CD34+ cell purity and an optimal depletion of T cells. The high rate of viral complications is probably due to the inability of residual T cells left from the CD34+ cell selection to generate, immediately after transplant, an adequate number of virus‐specific lymphocytes.