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IL‐10 increases the number of CFU–GM generatedby ex vivo expansion of unmanipulated human MNCsand selected CD34+ cells
Author(s) -
Wagner Thomas,
Fritsch Gerhard,
Thalhammer Renate,
Höcker Paul,
Lanzer Gerhard,
Lechner Klaus,
Geissler Klaus
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41050659.x
Subject(s) - ex vivo , leukapheresis , cord blood , progenitor cell , stem cell factor , cd34 , in vivo , immunology , cytokine , haematopoiesis , myeloid , andrology , bone marrow , stem cell , biology , chemistry , microbiology and biotechnology , medicine
BACKGROUND: Ex vivo expansion strategies with different cytokine combinations are currently used by several groups as a means of increasing the number of HPCs for a variety of special clinical applications. Because there is little information on the potential role of IL‐10 in such ex vivo expansion models, the effect of this cytokine on the generation of myeloid progenitor cells in suspension cultures was investigated. STUDY DESIGN AND METHODS: On the basis of data from the literature and from new experiments, the combination of SCF and IL‐3 at concentrations of 100 ng per mL and 100 U per mL, respectively, was chosen as the standard cocktail. The addition of IL‐10 to such cultures resulted in a marked and dose‐dependent potentiation of myeloid progenitor cell production. RESULTS: Using unmanipulated leukapheresis components from 13 individuals (including lymphoma and cancer patients and normal donors), the expansion multiple of CFU–GM after 14 days as compared with pre‐expansion values was 9.54 ± 2.31 times by SCF/IL‐3 and 46.38 ± 7.37 times by the combination of SCF/IL‐3 and 100 ng per mL of IL‐10 (p<0.001). IL‐10 also potentiated CFU–GM generation from selected CD34 PBMNCs (n = 9) with an expansion of 17.22 ± 7.04 times versus 45.67 ± 16.78 times using the SCF/IL‐3 and SCF/IL‐3/IL‐10 combination, respectively (p<0.05). Moreover, expansion‐promoting effects of IL‐10 were observed in liquid cultures containing MNCs from bone marrow (n = 4) and cord blood (n = 3), but did not reach statistical significance because of the small number of samples. CONCLUSION: These results suggest IL‐10 as a useful cytokine to optimize progenitor cell‐expansion strategies for clinical application.