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Evaluation of a whole‐blood WBC‐reduction filter that saves platelets: in vitro studies
Author(s) -
Larsson Stella,
Gulliksson Hans,
Paunovic Dragica
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41040534.x
Subject(s) - buffy coat , platelet , whole blood , hemolysis , filtration (mathematics) , chemistry , andrology , in vitro , mannitol , platelet poor plasma , chromatography , medicine , platelet rich plasma , immunology , biochemistry , statistics , mathematics
BACKGROUND: In this study, a new WBC‐reduction in‐line filter that removes WBCs but not platelets was evaluated. Three WBC‐reduced blood components were prepared: RBCs, plasma, and platelet concentrates (PCs). STUDY DESIGN AND METHODS: Whole‐blood components (n = 30) were filtered within 2 to 4 hours after collection and then were centrifuged and separated into RBCs, plasma, and WBC‐reduced buffy coat. Saline‐adenine‐glucose‐mannitol solution was added to the RBCs. The WBC‐reduced buffy coats were stored overnight; on the following day, PCs were prepared from pooled WBC‐reduced buffy coats and stored in a medium composed of approximately 35 percent CPD plasma and 65 percent platelet additive solution (T‐Sol, Baxter). The WBC‐reduction capacity of the filter, the recovery of cells after filtration, and the in vitro storage of RBCs (n = 10) and platelets (n = 6) were evaluated. RESULTS: Mean and maximum WBC counts after filtration were 0.08 × 10 6 and 0.3 × 10 6 , respectively, per filtered whole‐blood unit. Recovery of RBCs (mean values) after filtration was 90 percent in whole‐blood components and 73 percent in RBCs. Recovery of platelets (mean values) was 81 percent after filtration and 66 percent in PCs. The in vitro storage study of RBCs showed results comparable with previously published data, except for a lower degree of hemolysis. In the in vitro platelet storage study, results were compared with those of standard preparations. In all essentials, similar results were found. CONCLUSION: The results of the present study suggest that effective WBC reduction meets current standards and satisfactory recovery after filtration. The storage characteristics for RBCs and PCs are similar to those of standard preparations. Use of a whole‐blood in‐line filter to save platelets is a new option for whole‐blood processing, which may simplify WBC reduction and blood component preparation, as well as reduce costs in the future.