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In vitro and in vivo measurements of human RBCs frozen with glycerol and subjected to various storage temperatures before deglycerolization and storage at 4°C for 3 days
Author(s) -
Valeri C. Robert,
Pivacek Linda E.,
Cassidy George P.,
Ragno Gina
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41030401.x
Subject(s) - glycerol , hemolysis , blood preservation , sodium , cryopreservation , blood transfusion , in vivo , chemistry , surgery , andrology , medicine , biochemistry , biology , immunology , microbiology and biotechnology , embryo , organic chemistry
BACKGROUND: This study was designed to assess the effects of changes in storage temperature of frozen RBCs such as might occur during a malfunction of the −80°C mechanical freezer or during shipment. STUDY DESIGN AND METHODS: Fifteen participants donated blood for autologous transfusion of RBCs; all RBCs were frozen with 40‐percent (wt/vol) glycerol. Five subjects received RBCs that were stored at −80°C alone before transfusion. Five subjects received RBCs that were stored initially at −80°C, then at −40°C for 4 weeks, and finally at −80°C before transfusion. Five subjects received RBCs that were stored at −80°C, then at −20°C for 2 weeks, and finally at −80°C before transfusion. After deglycerolization, the RBCs were stored at 4°C in a sodium chloride‐glucose solution for 3 days before transfusion. RESULTS: No significant differences were observed in freeze‐thaw recovery, freeze‐thaw‐wash recovery, 24‐hour posttransfusion survival, index of therapeutic effectiveness, or RBC ATP levels. Greater hemolysis and reduced RBC K+ levels were observed in the units stored at −80°C/−40°C/−80°C and in those stored at −80°C/ −20°C/−80°C compared with the units stored at −80°C alone, but these differences did not affect the 24‐hour posttransfusion survival. CONCLUSIONS: The results of this study indicated that RBCs frozen with 40‐percent (wt/vol) glycerol can be stored at −40°C for 4 weeks or at −20°C for 2 weeks between periods of frozen storage at −80°C with satisfactory results.