Premium
The first example of a paraben‐dependent antibody to an Rh protein
Author(s) -
Judd W. John,
Storry Jill R.,
Annesley Thomas D.,
Reid Marion E.,
Bensette Michelle,
Waddington Sherry,
Dake LouAnn,
Rohrkemper David,
Valdez Ricardo
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41030371.x
Subject(s) - propylparaben , paraben , methylparaben , chemistry , antibody , chromatography , saline , microbiology and biotechnology , reagent , preservative , biology , immunology , organic chemistry , endocrinology
BACKGROUND: Parabens are added to a commercial LISS (C‐LISS) to retard microbial growth. Paraben‐dependent anti‐Jk a has been detected by the use of C‐LISS. CASE REPORT: Serum from a D+ woman reacted in antiglobulin tests with RBCs stored (2‐4 hours, 22‐25°C) in C‐LISS (Löw and Messeter formulation, Immucor). Freshly prepared C‐LISS‐suspended RBCs did not react; nor did RBCs stored in LISS‐additive reagents, PEG, saline, or homemade LISS. RESULTS: Studies using C‐LISS‐stored RBCs revealed an antibody that reacted with D+ and rrV+ RBCs, but not with r′r, r′′r, or rrV‐VS‐ RBCs. All partial D RBC phenotypes tested reacted, as did D+LW‐, r G r, r′′ G r, r y r, r′ s rV+VS+, and r′ s rV‐VS+ RBCs. The active ingredient in C‐LISS was propylparaben. Other LISS ingredients were not required; saline solutions of propylparaben, ethylparaben, methyl salicylate, 2‐phenoxyethanol, and butylparaben were active. Methylparaben and methyl‐ m ‐hydroxybenzoate were inactive. Reactivity to C‐LISS‐stored RBCs could not be inhibited by propylparaben. Reactivity with D+V‐ and D‐V+VS+ RBCs was not separable by adsorption‐elution. CONCLUSIONS: This antibody likely detects a neoantigen formed between active compounds and RBC membranes. Review of the structure of active compounds suggests that proximity between methyl and hydroxyl groups is important for binding with RBC membranes. The role of RhD is unclear; no single portion of RhD protein appears to be implicated.