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Cytokines and vascular cell adhesion molecule‐1 in the blood of patients undergoing HPC mobilization
Author(s) -
Dosquet Christine,
Chen Yolande,
Makke Jaffar,
Miclea JeanMichel,
Coudert MarieClaude,
Marolleau JeanPierre,
Fermand JeanPaul,
Cottu Paul,
Lotz JeanPierre,
Benbunan Marc
Publication year - 2001
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2001.41020206.x
Subject(s) - medicine , cell adhesion molecule , cd34 , cytokine , mobilization , leukapheresis , apheresis , granulocyte colony stimulating factor , plerixafor , immunology , oncology , chemotherapy , platelet , stem cell , receptor , biology , cxcr4 , chemokine , history , genetics , archaeology
BACKGROUND: The mechanism of HPC mobilization in humans is unclear. In this study, the relationship between PBPC mobilization and blood levels of G‐CSF, endogenous cytokines (IL‐8, SCF, thrombopoietin [TPO]), and the vascular cell adhesion molecule‐1 (VCAM‐1) was analyzed in patients with malignancy who were undergoing a PBPC mobilization regimen. STUDY DESIGN AND METHODS: Fifty‐four patients with multiple myeloma (MM) and 29 with breast cancer (BC) underwent a mobilization regimen combining conventional chemotherapy and G‐CSF up to the last day of PBPC collection. The CD34+ cell count was determined on each day when leukapheresis was scheduled. Venous blood samples (n = 117) were drawn before apheresis for CD34+ cell count (flow cytometry) and cytokine (G‐CSF, IL‐8, SCF, TPO) and VCAM‐1 measurements (ELISA). RESULTS: In multiple regression analysis, SCF was a significant determinant of CD34+ cell levels in BC patients (R = 0.50, p = 0.03) and of VCAM‐1 levels in MM patients (R = 0.32, p = 0.02). SCF was negatively correlated with CD34+ cell count in patients with BC. SCF and VCAM‐1 blood levels were correlated in MM and BC patients. CONCLUSION: SCF and VCAM‐1 could play a role in PBPC mobilization in patients and could be useful measures by which to study patients undergoing a mobilization regimen.