Premium
Human promyelocytic cell line: a convenient tool for studying the molecular basis of WBC filtration
Author(s) -
Barbe Laurent,
Boval Bernadette,
Wautier MariePaule,
Wautier JeanLuc
Publication year - 2000
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2000.40101250.x
Subject(s) - hl60 , filtration (mathematics) , cd11c , integrin alpha m , receptor , flow cytometry , chemistry , immunology , biology , cell , biochemistry , mathematics , statistics , gene , phenotype
BACKGROUND: Blood filtration is a technique widely used to reduce the levels of WBCs in blood components. Several studies have been conducted to define the factors that are involved in WBC reduction, but the various mechanisms are not clearly delineated. This study explored the role of WBC adhesion molecules in WBC reduction during filtration. STUDY DESIGN AND METHODS: A minifilter has been developed that has properties similar to those of the standard filter (Sepacell, Asahi Medical) but that allows a smaller volume of blood to be used (15 mL). WBC reduction was achieved to a similar extent in the standard filter and the minifilter (4.15 log and 4.18 log, respectively). Samples of human promyelocytic cell line (HL60) were filtered before and after differentiation induced by vitamin D3 (D3‐HL60). Flow cytometry was used to characterize the D3‐HL60 filtrates and to count the WBCs after filtration. RESULTS: HL60 was retained in the filter to the same extent as all other WBCs. A higher level of integrin receptors (CD11b/CD18; CD11c/CD18) was expressed by D3‐HL60 than by HL60. When the blood was incubated with anti‐CD11b, anti‐CD11c, or anti‐CD18, fewer D3‐HL60 cells were trapped by the filter, while only anti‐CD11b alters HL60 retention in the filter. CONCLUSION: The receptors CD11b/CD18 and CD11c/CD18 appear to bind to the filter fibers and to be one of the mechanisms responsible for WBC retention.