Premium
Variations in genes encoding neutrophil antigens NA1 and NA2
Author(s) -
Matsuo K.,
Procter J.,
Stroncek D.
Publication year - 2000
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2000.40060645.x
Subject(s) - gene , antigen , genotype , genetics , biology , microbiology and biotechnology
BACKGROUND: Neutrophil‐specific antigens NA1 and NA2 are located on Fcγ receptor IIIb (FcγRIIIb). NA1 and NA2 forms of FcγRIIIb differ by four amino acids and the corresponding genes by five nucleotides. Variations in NA gene frequencies are encountered among ethnic groups. Altered forms of the genes are expected among individuals. STUDY DESIGN AND METHODS: RFLPs associated with four recognition sites were used to determine NA genotypes of 232 individuals. When atypical NA genotypes were identified, FcγRIIIB and FcγRIIIA regions were sequenced. RESULTS: NA1 FcγRIIIB frequency in 100 Japanese (0.66) was greater than that in 53 African Americans (blacks) (0.40; p<0.01) and 79 whites (0.32; p<0.001). Sequencing of atypical FcγRIIIB in 16 people confirmed that four blacks had G→A substitutions at 227; 7 blacks had A→G substitutions at 277; and 1 Japanese person had C→G at 141 and G→T at 227. A at 227 and G at 277 represent expected nts of NA1 FcγRIIIB . One black had an NA1 FcγRIIIB with a G→A substitution at 349; A is normally found in NA2 FcγRIIIB at 349. Sequencing atypical FcγRIIIA in three persons revealed that two blacks had G→A substitutions at 277 plus C→A substitutions at 266 and 1 white had previously described T→G at 230. Two blacks with atypical NA2 FcγRIIIB had T→G FcγRIIIA at 230. One black was NA null . CONCLUSION : NA2 FcγRIIIB is more polymorphic in blacks than in whites or Japanese persons. Chimeric FcγRIIIB alleles are most similar to NA2 FcγRIIIB. One alternate allele of NA1 ( NA1*02 ) and four alternate alleles of NA2 ( NA2*02 , NA2*03 , NA2*04 , and NA2*05 ) are described.