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Effect of recombinant human megakaryocyte growth and development factor coupled with polyethylene glycol on the platelet storage lesion
Author(s) -
Snyder Edward L.,
Perrotta Peter,
Rinder Harvey,
Baril Laurene,
Nichol Janet,
Gilligan Diana
Publication year - 1999
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1999.39399219281.x
Subject(s) - platelet , megakaryocyte , mean platelet volume , chemistry , apheresis , thrombopoietin , polyethylene glycol , peg ratio , andrology , immunology , biochemistry , biology , medicine , haematopoiesis , microbiology and biotechnology , stem cell , finance , economics
BACKGROUND: Platelet production is regulated by a thrombopoietic growth factor (Mpl ligand).The receptor for this platelet growth factor (Mpl) is expressed on the platelet surface membrane. A recombinant thrombopoietic cytokine, recombinant human megakaryocyte growth and development factor coupled with polyethylene glycol (PEG‐rHuMGDF), was added to apheresis platelets in vitro to determine whether Mpl ligand–receptor binding produced any beneficial or adverse effect on the development of the platelet storage lesion during 5 days of storage. STUDY DESIGN AND METHODS: This study was designed as a dose‐response protocol to determine the effects of adding increasing concentrations of PEG‐rHuMGDF (0.0 [control], 2.5, 25, and 250 ng/mL) to apheresis platelets stored in two types of plastic storage containers. The increasing concentrations of PEG‐rHuMGDF used simulated the theoretical peak plasma level attained in vivo, with an intravenous dose of 0, 0.1, 1.0 and 10 μg per kg of PEG‐rHuMGDF. The platelets were stored with agitation at 20 to 24°C for 5 days. A battery of in vitro assays was performed on storage Days 1 and 5, including pH, blood gases, platelet count, lactate dehydrogenase, mean platelet volume, glucose, lactate, osmotic recovery, morphology score, CD62P, and one‐dimensional polyacrylamide gel electrophoresis analyses. RESULTS: Analysis of results on both Day 1 and Day 5 showed no significant differences among any of the three PEG‐rHuMGDF doses and the control group, for any in vitro assay. One‐dimensional polyacrylamide gel electrophoresis showed no changes among the platelet protein patterns for the three PEG‐rHuMGDF doses studied or the control. Storage‐induced changes, however, did occur equally in all four groups of platelets over the 5 days of storage. CONCLUSION: The addition to stored apheresis platelets of up to 10 μg per kg of PEG‐rHuMGDF (250 ng/mL), followed by 5 days of storage at standard conditions, does not appear to promote or retard development of the platelet storage lesion.