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Simultaneous genotyping of human platelet antigens (HPA) 1 through 6 using new sequence‐specific primers for HPA‐5
Author(s) -
Meyer O.,
Hildebrandt M.,
Schulz B.,
Blasczyk R.,
Salama A.
Publication year - 1999
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1999.39111256.x
Subject(s) - genotyping , sequence (biology) , biology , genetics , antigen , computational biology , platelet , immunology , genotype , gene
BACKGROUND: Polymerase chain reaction using sequence‐specific primers is widely used for genotyping human platelet antigens (HPA). However, the results of HPA‐5 genotyping are still problematic. STUDY DESIGN AND METHODS: New sequence‐specific primers were designed for HPA‐5 that, together with already published primers, allow simultaneous genotyping of HPA‐1, ‐2, ‐3, ‐4, ‐5 , and ‐6 . The reliability of the described protocol was determined by using reference DNA samples as well as samples from healthy blood donors. RESULTS: All primers produced specific amplification products. The genotype and the previously ascertained phenotype of the tested specimens were in concordance in all cases. CONCLUSION: The described polymerase chain reaction protocol allows rapid, reliable, simultaneous genotyping of HPA‐1, ‐2, ‐3, ‐4, ‐5 , and ‐6 .