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Application of RHD and RHCE genotyping for correct blood group determination in chronically transfused patients
Author(s) -
Legler T.J.,
Eber S.W.,
Lakomek M.,
Lynen R.,
Maas J.H.,
Pekrun A.,
RepasHumpe M.,
Schröter W.,
Köhler M.
Publication year - 1999
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1999.39080852.x
Subject(s) - genotyping , serology , typing , medicine , blood transfusion , immunology , genotype , biology , genetics , antibody , gene
BACKGROUND: In chronically transfused patients, conventional blood group typing may be impossible because of mixed‐field agglutination. STUDY DESIGN AND METHODS: In 27 patients with congenital anemia and lifelong transfusion history, genotyping for D, RHD , and RHCE was performed with polymerase chain reactions. These results were compared with the blood group typing results documented in the medical record. RESULTS: Two of 27 cases had been typed D‐negative by serologic tests and D‐positive by genotyping. In 20 patients, the CDE formula had been determined serologically according to the medical record; 4 of these patients were Cc by serologic tests and C/C by genotyping. One patient typed ee by serologic tests, and genotyping revealed heterozygosity (E/e) . CONCLUSION: In patients with a lifelong transfusion history, serologic blood group determination may be impossible, and pretransfusion test results are not always available or reliable. In whites, Rh‐matched transfusions are possible with genotyping. The genetic background of the RH genes has to be elucidated in other ethnic groups, such as in black patients with sickle cell disease, before genotyping can be applied without restriction.