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Bacterial contamination rates following processing of bone marrow and peripheral blood progenitor cell preparations
Author(s) -
Padley D. J.,
Koontz F. P.,
Trigg M. E.,
Gingrich R. D.,
Strauss R. G.
Publication year - 1996
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1996.36196190515.x
Subject(s) - progenitor cell , bone marrow , medicine , contamination , sepsis , peripheral blood , blood cell , stem cell , immunology , biology , ecology , genetics
Background: The performance of cultures to assess possible bacterial contamination of bone marrow and peripheral blood progenitor cell preparations is required by the standards of the American Association of Blood Banks. Study Design and Methods: Consecutive (n = 893) bone marrow and peripheral blood progenitor cell preparations were cultured for assessment of possible contamination by microorganisms. Results: Consecutive bone marrow and peripheral blood progenitor cell preparations (n = 893) were cultured; the overall positive rate detected was 2.5 percent (22/893). The isolates predominantly were skin contaminants (gram‐positive cocci) and so‐called water‐borne organisms (gram‐negative rods). The 6.0‐percent rate of positivity in 317 bone marrow preparations was higher than the 0.5‐percent rate in 576 peripheral blood progenitor cell preparations (p < 10(‐6)). Culture‐ positive preparations were transfused to 16 patients at this institution; however, none of these transfusions led to documented sepsis with the contaminating organism. Conclusion: The culture method described here complies with the standards of the American Association of Blood Banks. Contamination can be detected in both bone marrow and peripheral blood progenitor cell preparations. When contaminated preparations are transfused, there are few complications that can be attributed to the contamination.