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Buffy coat platelets stored in apyrase, aprotinin, and ascorbic acid in a suspended bag: combined strategies for reducing platelet activation during storage
Author(s) -
Mrowiec Z. R.,
Oleksowicz L.,
Zuckerman D.,
LeonFernandez M.,
Khorshidi M.,
Dutcher J. P.,
Puszkin E. G.
Publication year - 1996
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1996.36196190509.x
Subject(s) - buffy coat , platelet , apyrase , chemistry , ascorbic acid , aprotinin , andrology , platelet activation , medicine , immunology , food science
Background: Platelet activation is an important factor impeding the clinical effectiveness of platelet transfusions. In this study, platelet concentrates (PCs) were prepared by a novel suspended‐bag buffy coat technique that was followed by the addition of a mixture of platelet activation inhibitors to the storage bag. Study Design and Methods: In vitro platelet function was evaluated in PCs prepared by the suspended‐bag buffy coat technique and stored at 22 degrees C for 5 days in the presence of (n = 12) or absence (n = 12) of apyrase, ascorbic acid, and aprotinin (AAA). Results: Platelets from AAA‐ incubated PCs demonstrated mean ATP levels 17 percent (p < 0.004), 13 percent (p < 0.02), and 22 percent (p < 0.003) higher than those measured in parallel control PCs on Days 1, 3, and 5, respectively. Similarly, on Days 3 and 5 of storage, respectively, 45‐percent (p < 0.001) and 50‐percent (p < 0.001) greater ADP‐induced maximum aggregation was observed in AAA‐incubated PCs than was seen in control preparations. AAA‐incubated PCs demonstrated alpha‐granule membrane protein‐140 expression 92 percent (p < 0.01), 133 percent (p < 0.003), and 104 percent (p < 0.001) below that in control PCs on Days 1, 3, and 5, respectively. At similar intervals, a significant increase in recovery from hypotonic shock also was observed in AAA‐incubated PCs. Further, Day 5 AAA‐PCs demonstrated significantly higher morphology scores and O2 consumption than did control preparations. Conclusion: Buffy coat platelets prepared in suspended bags and stored in the presence of AAA demonstrate significantly reduced activation and enhanced functional and metabolic activity.