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White cell‐reduced platelet concentrates prepared by in‐line filtration of platelet‐rich plasma
Author(s) -
Sweeney J. D.,
Holme S.,
Heaton W. A. L.,
Nelson E.
Publication year - 1995
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1995.35295125735.x
Subject(s) - platelet , filtration (mathematics) , platelet rich plasma , white blood cell , whole blood , mean platelet volume , chemistry , andrology , chromatography , medicine , immunology , mathematics , statistics
BACKGROUND: The importance of white cell (WBC) reduction in platelet concentrates (PCs) for component quality is undetermined. STUDY DESIGN AND METHODS: Eleven paired components, each derived from one of two whole‐blood units given by a single donor on the same day, were studied. One PC was WBC reduced by filtration with an in‐line, integral, prototype filter, and the other was produced from unfiltered platelet‐rich plasma (PRP) by a standard method. In vitro tests performed on Day 1 and Day 5 were blood gases, plasma lactate, glucose, platelet ATP, mean platelet volume, morphology score, hypotonic stress ratio, extent of shape change in response to ADP, and beta‐ thromboglobulin. After 5 days of storage, each component pair was labeled with 51Cr or 111In and transfused for the estimation of percent recovery and survival. RESULTS: PCs using the in‐line, prototypic filter had a platelet loss of approximately 15 percent and a variable 1 to 3 log10 reduction (average, 95%) in WBC content. The variation in filter WBC removal was related to PRP WBC content and indicated that the filter did not have the capacity for a 3 to 4 log10 removal when PRP WBC content exceeded 1 × 10(8). The in vitro and in vivo measures of platelet quality showed no meaningful differences between filtered and unfiltered PCs by paired t test. The mean differences in posttransfusion percent recoveries and survivals were 0.9 +/− 2.9 percent and 4 +/− 13 hours, respectively. Additional studies were performed using a larger filter with improved capacity. Those studies (n = 18) showed a significant improvement in filtration time and platelet yield and a consistent 3 to 4 log10 reduction in WBCs. Filtration time was 6.6 +/− 2.7 minutes, total PC WBCs were 9.6 +/− 4.6 × 10(4), and total PC platelets were 7.8 +/− 1.8 × 10(10) (mean +/− SD). CONCLUSION: Prestorage filtration of PRP and the preparation of filtered platelets do not result in any significant beneficial or adverse effect on subsequent platelet quality. With the large‐capacity filter, consistent WBC reduction and good platelet yields are achieved.