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Comparative analysis of six different white cell‐reduction filters for packed red cells
Author(s) -
Bontadini A.,
Fruet F.,
Tazzari P.L.,
Lollini P.L.,
Conte R.
Publication year - 1994
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1994.34694295071.x
Subject(s) - flow cytometry , red cell , medicine , andrology , chemistry , immunology
Background : The reduction of white cells in blood components before transfusion by filters with at least 3 log 10 depletion may prevent adverse transfusion reactions such as HLA alloimmunization, febrile reactions, transmitted infections, and immunomodulation. A new generation of filters with 4 log10 depletion is now available. Study Design and Methods : The aim of this study is to compare the efficiency of white cell reduction by six commercial filters for packed red cells with 3 and 4 log10 depletion (claimed by manufacturers). The analysis of white cell concentration in the white cell‐reduced units was performed by flow cytometry and with a Nageotte chamber. Results : The last generation of filters (BPF4, RC400, R01 Plus) show mean residual white cell numbers of 0.18 ± 0.14, 0.26 ± 0.21, and 0.25 ± 0.15 × 10 6 , respectively, by flow cytometric analysis and 0.05 ± 0.04, 0.18 ± 0.15, and 0.38 ± 0.23 × 10 6 , respectively, by Nageotte chamber evaluation. The 3 log 10 depletion filters (R01, Leucostop‐4LT‐ mono, R200) have mean residual white cell numbers of 1.41 ± 0.92, 2.4 ± 1.99, and 1.05 ± 0.64 × 10 6 , respectively, by flow cytometric analysis and 3.56 ± 1.7, 1.67 ± 1.3, and 3.21 ± 4.1 × 10 6 , respectively, by Nageotte chamber evaluation. The data show that the BPF4, RC400, and R01 Plus filters are likely to be more efficient by 1 log 10 reduction than the R01, Leucostop‐4LT‐mono, and R200 filters. Conclusion : The most recent generation of filters is able to deplete white cells from packed red cells by 4 log 10 ; in particular, with one of the filters, the residual WBC content was less than 0.5 × 10 6 per unit in all experiments, while two other filters reached that level in 9 of 10 experiments.