Premium
Altered expression of adhesion molecules (L‐selectin and Mac‐1) on granulocytes during storage
Author(s) -
Wikman A.,
Lundahl J.,
Fernvik E.,
Shanwell A.
Publication year - 1994
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1994.34294143948.x
Subject(s) - granulocyte , receptor , sepsis , adhesion , cell adhesion molecule , selectin , andrology , chemistry , receptor expression , l selectin , immunology , microbiology and biotechnology , medicine , biochemistry , biology , organic chemistry
Background: The success of granulocyte transfusion therapy for neutropenic patients with sepsis is dependent on the number and quality of the granulocytes transfused. There is a progressive impairment in granulocyte function during storage. Study Design and Methods: The effect of 1 to 2, 4, 24, and 48 hours' storage on receptor expression associated with granulocyte function has been analyzed. Results: After 24 hours' storage, significant changes were found in the expression of receptors associated with adhesion to the endothelium: a decrease in L‐ selectin expression (p < 0.01) and an increase in Mac‐1 expression (p < 0.01). Receptors (CR1 and FcRIII), associated with adhesion to target, were either increased (CR1) or unaltered (FcRIII). The capacity to produce a reactive oxygen metabolite (hydrogen peroxide) remained essentially unchanged after 48 hours' storage. The ability of N‐formyl‐ methionyl‐leucyl‐phenyl alanine to mobilize Mac‐1 and CR1 was reduced after 48 hours' storage. Conclusion: Since the regulation of adhesion molecules is important for the recruitment of granulocytes into an inflammatory site, the observed in vitro changes in L‐selectin and Mac‐ 1 expression during storage may be of importance for the quality of granulocyte concentrates.