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Hematopoietic progenitor cells are resistant to dimethyl sulfoxide toxicity
Author(s) -
BRANCH D.R.,
CALDERWOOD S.,
CECUITI M.A.,
HERST R.,
SOLH H.
Publication year - 1994
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1994.341095026975.x
Subject(s) - dimethyl sulfoxide , megakaryocyte , haematopoiesis , progenitor cell , bone marrow , colony forming unit , granulocyte , toxicity , peripheral blood mononuclear cell , immunology , macrophage , andrology , cfu gm , chemistry , microbiology and biotechnology , biology , stem cell , medicine , biochemistry , in vitro , genetics , organic chemistry , bacteria
BACKGROUND: A direct chemical toxicity of dimethyl sulfoxide (DMSO) to hematopoietic progenitor cells has been suggested. However, a recent study failed to corroborate these earlier findings. Thus, a series of experiments was undertaken to address this issue. STUDY DESIGN AND METHODS: Bone marrow was collected from 18 donors and cryopreserved with 10 percent (vol/vol) DMSO. Aliquots of frozen bone marrow were thawed, diluted with ACD‐A to 8 percent (vol/vol) DMSO, and allowed to remain in DMSO for up to 2 hours before mononuclear cells were plated for colony‐forming assays. After 14 days in culture, burst‐forming units‐erythroid, colony‐forming units–granulocyte/macrophage, and colony‐forming units–granulocyte/erythrocyte/macrophage/megakaryocyte colonies were enumerated. RESULTS: There was no significant difference (p > 0.5) seen in colony formation over the 2‐hour exposure to DMSO. CONCLUSION: These results support and extend a previous study that bone marrow hematopoietic progenitor cells, including burst‐forming units–erythroid, colony‐forming units–granulocyte/macrophage, and colony‐ forming units–granulocyte/erythrocyte/macrophage/megakaryocyte are resistant to any toxic effects of 8‐ to 10‐percent (vol/vol) DMSO during at least 2 hours of DMSO exposure.