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A flow cytometric method for counting very low levels of white cells in blood and blood components
Author(s) -
Vachula M,
Simpson S.J.,
Martinson J.A.,
Aono F.M.,
Hutchcraft A.M.,
Balma D.L.,
Epps D.E.
Publication year - 1993
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1993.33393174454.x
Subject(s) - flow cytometry , blood flow , medicine , white (mutation) , immunology , biology , genetics , gene
Reduction of white cells (WBCs) in blood components may reduce the risk of virus transmission and HLA alloimmunization. Filtration provides a means by which to achieve high‐efficiency WBC reduction. A method has been developed using flow cytometry to quantitate the number of WBCs in WBC‐reduced packed red cells or platelet concentrates. This method uses a detergent and propidium iodide (PI) solution to label the WBC nuclei and incorporates a known amount of fluorescein isothiocyanate (FITC)‐ labeled chicken red cells (cRBCs) into the mixture as an indicator of the volume examined. The number of observed WBCs per mL is calculated as follows: Number of PI WBC nuclei events/Number of FITC cRBC events × Number of FITC cRBCs added to mixture/Volume of blood in mixture. The method may allow the detection of WBCs at a concentration as low as 0.01 per microliters (10/mL) in a blood sample. It is an efficient method of collecting data, as it requires less than 10 minutes per sample. This flow cytometric technique is suitable for research purposes and for quality control of WBC‐reduced blood components, because it is precise and can be used to quantitate WBCs in large or small numbers in a sample.