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Direct identification of Yersinia enterocolitica in blood by polymerase chain reaction amplification
Author(s) -
FENG P.,
KEASLER S.P.,
HILL W.E.
Publication year - 1992
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1992.32993110759.x
Subject(s) - yersinia enterocolitica , polymerase chain reaction , plasmid , biology , gene , microbiology and biotechnology , enterobacteriaceae , yersinia , bacteremia , bacteria , genetics , escherichia coli , antibiotics
Primers based on the nucleotide sequence of the virF gene in the pYV plasmid and the chromosomal ail gene were used in polymerase chain reaction (PCR) amplifications to directly identify Yersinia enterocolitica in blood. Approximately 500 bacteria seeded into 100 microL of blood can be extracted and amplified by PCR to yield positive results. PCR analyses of seven Y. enterocolitica isolates previously implicated in blood contaminations showed that only one isolate harbored the plasmid‐borne virF gene; however, all seven isolates were identified effectively by the PCR product amplified from the chromosomal gene. The PCR assay has the potential for use in the identification of Y. enterocolitica contamination in stored units of blood or in the rapid diagnosis of transfusion‐related bacteremia caused by Y.