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Removal of Yersinia enterocolitica from AS‐1 red cells
Author(s) -
Buchholz D.H.,
AuBuchon J.P.,
Snyder E.L.,
Kandler R.,
Edberg S.,
Piscitelli V.,
Pickard C.,
Napychank P.
Publication year - 1992
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1992.32792391043.x
Subject(s) - yersinia enterocolitica , inoculation , colony forming unit , microbiology and biotechnology , biology , enterobacteriaceae , bacteria , zoology , food science , immunology , escherichia coli , biochemistry , genetics , gene
The growth of Yersinia enterocolitica in AS‐1 red cells was investigated so as to study the organism's proliferation kinetics and to evaluate the effect of prestorage white cell (WBC) reduction on bacterial multiplication. Twenty‐four 2‐unit pools of ABO‐compatible whole blood were prepared and inoculated with Y. enterocolitica to final concentrations ranging from 0.3 to 132 organisms per mL. After inoculation, pools were split equally, AS‐1 red cells were prepared, and 1 unit of each pair (test unit) was WBC‐reduced with a WBC‐ reduction filter. Quantitative bacterial cultures of both WBC‐reduced and control units were performed at several points throughout preparation and storage. Less than 10 percent of the inoculated organisms was recovered from blood samples taken after a 7‐hour room‐ temperature holding period. By the end of 42 days of storage, Y. enterocolitica was recovered from unfiltered red cells in 2 of the 6 units inoculated at the lowest levels (0.3 and 0.7 organism/mL), from 8 of the 12 units inoculated at the intermediate levels (2.8, 5.2, 30.7, and 43 organisms/mL) and from 6 of the 6 units inoculated at the highest levels (98.8 and 132 organisms/mL). Positive cultures were seen as early as Day 7. In contrast, filtered units inoculated at all levels ≥ to 98.8 organisms per mL (21/21 units) were sterile at the end of the 42‐day storage period, while 2 units (2/3) inoculated at 132 organisms per mL showed growth despite filtration. Thus, passage of deliberately contaminated AS‐1 red cells tarough this filter appeared to reduce significantly the likelihood of growth of Y. enterocolitica during subsequent storage, in spite of the removal of large numbers of WBCs. Because the concentration of bacteria in asymptomatic carrier donors is thought to be very low, prestorage filtration of blood shortly following col‐ lection may represent a means by which the risk of subsequent proliferation of Y.enterocolitm may be reduced or avoided as well as a means of providing a WBC‐ reduced blood component.