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Detection of antibodies to human immunodeficiency virus type 2 (HIV‐2) in blood donor sera using United States assay methods for anti‐HIV type 1
Author(s) -
Sazama K.,
Kuramoto I.K.,
Holland P.V.,
Couroucé AM.,
Gallo D.,
Hanson C.V.
Publication year - 1992
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1992.32592327710.x
Subject(s) - virology , human immunodeficiency virus (hiv) , antibody , blood type (non human) , medicine , blood donor , virus , immunology , abo blood group system
Twelve serum samples from French blood donors that were uniformly reactive in tests for antibody to human immunodeficiency virus type 2 (anti‐HIV‐2) also were reactive in 92 to 100 percent of tests with three anti‐HIV type 1 (anti‐HIV‐1) enzyme‐linked immunoassays currently in widespread use for donor screening in the United States. Supplemental tests for anti‐HIV‐1 on these anti‐HIV‐2‐reactive samples differed in their responses. All samples reacted in a licensed anti‐HIV‐1 Western blot, but there was an atypical band near the p41 position, which could be a clue to the fact that this result was a cross‐reaction with anti‐HIV‐2. A recombinant immunoblot gave an indeterminate result for anti‐HIV‐1 in all 12 samples. A local immunofluorescence assay for anti‐HlV‐1 reacted with 92 percent of the samples, but a commercial one detected only 58 percent.