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In vitro and in vivo evaluation of cotton wool filtration of platelet concentrates obtained by automated and manual apheresis
Author(s) -
HOLME S.,
SNYDER E.,
HEATON A.,
KEEGAN T.,
NAPYCHANK P.
Publication year - 1992
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1992.32492263446.x
Subject(s) - apheresis , wool , filtration (mathematics) , platelet , platelet concentrate , blood product , in vivo , chemistry , medicine , surgery , immunology , biology , microbiology and biotechnology , materials science , mathematics , composite material , statistics
The effect of cotton wool filtration of apheresis platelet concentrates (PCs) on platelet viability and complement activation was evaluated by two laboratories. PCs were prepared by automated (Lab A, n = 5) or manual (Lab B, n = 5) apheresis. After storage for 1 day, the PC was filtered through cotton wool before transfusion on one occasion and, on the other occasion, filtered through a standard screen filter before transfusion to the same donor. Five paired studies were performed by each laboratory. Except for a small, but significant reduction in mean platelet size, from 7.3 +/− 1.1 to 6.6 +/− 0.9 microns 3, after cotton wool filtration, no effect of filtration on various tests of in vitro platelet function and morphologic integrity was found. As demonstrated by autologous radiolabeled studies, no effect of cotton wool filtration on platelet viability was found by Laboratory B, while Laboratory A found a slight increase in the percentage of recovery from 59 +/− 4 to 68 +/− 13 percent, and a small reduction in survival, from 8.2 +/− 0.9 to 7.7 +/− 0.5 days after cotton wool filtration (p less than 0.05). Cotton wool filtration was associated with a slight increase in C3a levels found in manual apheresis PCs. Neither laboratory found any effect of cotton wool filtration per se on the recipients' white cell (WBC) counts or C3a and C5a levels after transfusion. However, it was observed that the transfusion of automated PCs (whether filtered through cotton wool or not filtered) caused a small, but significant (p<0.05; F<0.05) drop of 13 percent i recipient WBC count after transfusion, which is indicative of some in vivo effect from the automated apheresis procedure. These studies show that WBC reduction by the cotton wool filtration of apheresis PCs does not cause any significant damage to the platelets or activate the recipient complement reaction, as measured by recipient posttransfusion levels of WBCs, C3a, or C5a.