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Platelet storage in synthetic media lacking glucose and bicarbonate
Author(s) -
Murphy S.,
Kagen L.,
Holme S.,
Gottlieb B.,
Heaton W.,
Grode G.,
Davisson W.,
Buchholz D.
Publication year - 1991
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1991.31191096178.x
Subject(s) - bicarbonate , platelet , lactic acid , chemistry , sodium bicarbonate , in vivo , platelet concentrate , sodium citrate , platelet rich plasma , biochemistry , chromatography , medicine , bacteria , biology , microbiology and biotechnology , organic chemistry , pathology , genetics
The goal of these studies was to develop a cost‐effective medium for the storage at 22 +/− 2 degrees C of platelets for transfusion. Platelet concentrates were prepared from platelet‐rich plasma in a standard fashion and resuspended in 55 mL of medium. Residual plasma was 14.4 +/− 4.3 percent of the final suspending solution. The use of a simple solution containing electrolytes and citrate, but no glucose or bicarbonate, was associated with a drop in pH to the range of 6.0 to 6.6, as a result of the production of lactic acid from the platelet glycogen and glucose present in residual plasma. When 25 mM (25 mmol/L) sodium phosphate was included as a buffer, the drop in pH was retarded, but the range throughout storage was still 6.5 to 6.8. Nonetheless, platelets stored for 5 days in this phosphate‐containing medium showed satisfactory maintenance of many in vitro variables. However, there was a 10 to 35 percent reduction of in vivo recovery after isotopic labeling. There was no significant reduction in subsequent mean cell life. Because of the in vivo abnormalities, these media are not recommended for use at the present time. However, the results are encouraging and suggest that further research may lead to a satisfactory, cost‐effective medium.