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The use of tri(n‐butyl)phosphate detergent mixtures to inactivate hepatitis viruses and human immunodeficiency virus in plasma and plasma's subsequent fractionation
Author(s) -
Piët M.P.J.,
Chin S.,
Pprince A.M.,
B.rotman B.,
Cundell A.M.,
Horowitz B.
Publication year - 1990
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1990.30790385516.x
Subject(s) - virology , virus , blood proteins , albumin , chemistry , medicine , chromatography , biochemistry
The treatment of plasma with organic solvent/detergent mixtures at the time of plasma collection or pooling could reduce the exposure of technical staff to infectious viruses and enhance the viral safety of the final product. Treatment of plasma for 4 hours with 2‐percent tri(n‐ butyl)phosphate (TNBP) at 37°C, with 1‐percent TNBP and 1‐ percent polyoxyethylensorbitan monooleate (Tween 80) at 30°C, or with 1‐percent TNBP and 1‐percent polyoxyethylene ethers, (Triton X‐ 45) at 30°C resulted in the rapid and complete inactivation of ≥ 10 4 tissue culture‐infectious doses (TCID 50 ) of vesicular stomatitis and Sindbis viruses, which are used as surrogates. Treatment of plasma with TNBP and TNBP and Tween‐80 was shown to inactivate ≥ 10 4 TCID 50 of human immunodeficiency virus. TNBP treatment of plasma contaminated with 10 6 chimpanzee‐infectious doses (CID 50 ) of hepatitis B virus and 10 5 CID 50 of non‐A, non‐B hepatitis virus prevented the transmission of hepatitis to chimpanzees. Immediately after treatment of plasma with 2‐percent TNBP, the recovery of factors VIII, IX, and V and antithrombin III was 80, 90, 40, and 100 percent, respectively. Recovery of all factors was ≥ 90 percent after treatment with TNBP and detergent mixtures. Treated plasma was fractionated by standard techniques into antihemophilic factor and prothrombin complex concentrates, immune globulin, and albumin. Prior treatment with TNBP or TNBP and detergent did not affect the separations of desired proteins. Therefore, it appears possible to inactivate viruses in plasma before the execution of standard fractionation procedures.