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A METHOD FOR COUNTING LEUKOCYTES IN FILTERED COMPONENTS
Author(s) -
GREENWALT TJ,
ALLEN CM
Publication year - 1990
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1990.30490273449.x
Subject(s) - hemocytometer , computer science , simple (philosophy) , chromatography , reliability engineering , medicine , chemistry , engineering , pathology , philosophy , epistemology
Leukocyte‐depleted components are being used more frequently. Quantitation of residual WBCs is needed for quality control and for establishing the critical level required to prevent alloimmunization. Electronic counting is not accurate at WBC levels below 100/μL (1) and previously proposed techniques are either tedious or require sophisticated equipment. We describe a simple hemacytometer procedure using Türks solution that gives 87–93% recovery at WBC levels below 40/μL.