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Effects of room‐temperature exposure on bacterial growth in stored red cells
Author(s) -
Hamill T.R.,
Hamill S.G.,
Busch M.P.
Publication year - 1990
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1990.30490273435.x
Subject(s) - staphylococcus epidermidis , bacterial growth , yersinia enterocolitica , citrobacter freundii , microbiology and biotechnology , bacteria , colony forming unit , biology , citrobacter , food science , bacterial colony , contamination , streptococcus , enterobacter , staphylococcus aureus , enterobacteriaceae , escherichia coli , ecology , biochemistry , gene , genetics
Experiments were performed to determine the effect of room‐temperature (RT) exposure on the growth rates of selected bacteria by deliberate contamination of AS‐1 packed red cell (RBC) units contained in PL146 plastic bags. Isolates of Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Pseudomonas aeruginosa, Citrobacter freundii, Enterobacter agglomerans , and Yersinia enterocolitica were inoculated into 3‐ to 5‐day‐old packed RBC units. The units were refrigerated at 4 to 6° C for 14 days. On two occasions during this period, one‐half of the units were removed from the refrigerator and held at 25 to 26° C for 2 hours. Timed samples taken during the periods at RT revealed stable or decreasing bacterial counts in the majority of exposed units, and in no instance was a marked increase in bacterial growth observed. Samples taken after subsequent prolonged rerefrigeration showed no significant increase in bacterial counts as compared with those in control units that were refrigerated constantly. These results indicate that RT exposure of contaminated RBC units does not result in accelerated bacterial proliferation over a period of 2 hours and that it may, in fact, have a detrimental effect on bacterial growth.