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Flow cytometric studies of the D antigen of various Rh phenotypes with particular reference to D u and D el
Author(s) -
Hasekura H.,
Ota M.,
Ito S.,
Hasegawa Y.,
Ichinose A.,
Fukushima H.,
Ogata H.
Publication year - 1990
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1990.30390194344.x
Subject(s) - antigen , genotype , microbiology and biotechnology , phenotype , flow cytometry , immunofluorescence , chemistry , biology , antibody , gene , immunology , genetics
Flow cytometric analysis was performed on red cells (RBCs) to compare the antigen activity of various Rh phenotypes, including the rare variants D el and D u (both high‐grade and low‐grade), whose genotypes were confirmed or presumed by the family study. This appears to be the first report that the D el phenotype is due to the inhibitory effect of Cde. This study also compares the quantity of the D antigen in different phenotypes, whose genotypes comprise the same D u gene ( cD u E ) transmitted in a family. RBCs were stained by the indirect immunofluorescence method using IgG anti‐D purified through a protein‐A affinity column. The mean fluorescence intensity obtained for each genotype representing the activity of the D antigen decreased in the following order (percentages show relative fluorescence intensity taking cDE/cDE control as 100% and Cde/cde as 0%): CDe/cDE , 76 percent; CDe/cde , 73 percent; cDE/Cde , 70 percent; cDE/cD u E , 68 percent; CDe/Cde (high‐grade D u ), 59 percent; cD u E/cde (low‐grade D u ), 6 percent; and cD u E/Cde (D el ), 3 to 4 percent.