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Accurate quantitation of the low number of white cells in white cell‐depleted blood components
Author(s) -
Kao K.J.,
Scornik J.C.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29990070180.x
Subject(s) - propidium iodide , white blood cell , staining , blood cell , stain , andrology , immunology , red blood cell , microbiology and biotechnology , biology , chemistry , medicine , pathology , apoptosis , biochemistry , programmed cell death
A simple method of accurate quantification of low concentrations of white cells (WBCs) in WBC‐depleted blood components was developed by using propidium iodide (PI) to stain the nuclei of WBCs. The method was validated by correlating the PI‐determined WBC concentrations with those determined with Coulter S‐plus IV counter in units of packed red cells (PRBCs) or random‐donor platelets (RDPs). The correlations were linear and had coefficients of 0.99. The sensitivity of PI staining permitted the detection of concentrations of WBCs as low as 1 cell per μL of RDPs or 11 cells per μL of PRBCs. Therefore, PI staining will be useful in investigating the role of transfused WBC‐depleted blood components in the prevention of primary alloimmunization to HLA antigens, as well in evaluating various new procedures with high efficiency in depleting WBCs from blood components.